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Related Experiment Videos

Regulatory volume decrease by cultured renal cells.

C Knoblauch1, M H Montrose, H Murer

  • 1Institute of Physiology, University of Zurich, Switzerland.

The American Journal of Physiology
|February 1, 1989
PubMed
Summary
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Opossum kidney (OK) cells exhibit regulatory volume decrease (RVD) when exposed to hypotonic stress. This cell volume regulation involves specific ion channels, particularly potassium (K+) and chloride (Cl-) channels.

Area of Science:

  • Cell biology
  • Physiology
  • Biochemistry

Background:

  • Cell volume regulation is crucial for cellular function.
  • Epithelial cells, like those from the kidney, have specific mechanisms to maintain volume homeostasis.
  • Understanding these mechanisms is key to understanding kidney function and disease.

Purpose of the Study:

  • To investigate the volume regulatory responses of opossum kidney (OK) cells.
  • To elucidate the ionic mechanisms underlying regulatory volume decrease (RVD) in OK cells.
  • To identify the specific ion channels involved in RVD.

Main Methods:

  • Electronic cell sizing to measure cell volume changes.
  • Intracellular pH measurements in cell suspensions.
  • Manipulation of extracellular ion concentrations (Cl-, acetate, NO3-).

Related Experiment Videos

  • Inhibition studies using quinine, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), and valinomycin.

    • Main Results:

    • OK cells demonstrated a regulatory volume decrease (RVD) following hypotonic swelling.
    • RVD was modulated by extracellular Cl- concentration, with acetate reducing and NO3- accelerating the response.
    • Quinine and DIDS inhibited RVD, suggesting involvement of ion channels.
    • Valinomycin also inhibited RVD, further supporting the role of K+ channels.
    • DIDS's inhibitory effect was dependent on the presence of Cl-.

    Conclusions:

    • The RVD response in OK cells is mediated by the activation of distinct K+ and Cl- channels.
    • These findings provide insights into the molecular mechanisms of cell volume regulation in renal epithelia.
    • The study highlights the importance of ion transport in maintaining cellular homeostasis.