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Related Experiment Videos

A simple device for rapidly exchanging solution surrounding a single cardiac cell.

K W Spitzer1, J H Bridge

  • 1Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City 84112.

The American Journal of Physiology
|February 1, 1989
PubMed
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Researchers developed a novel device for rapid solution switching around cardiac cells, enabling faster physiological studies. This innovation allows precise control of the cellular environment, crucial for understanding cell function.

Area of Science:

  • Cardiovascular Physiology
  • Cellular Electrophysiology
  • Biomedical Engineering

Background:

  • Studying cardiac cell function requires precise control over the extracellular environment.
  • Rapid solution exchange is critical for accurately measuring cellular responses to stimuli.
  • Existing methods may not offer sufficient speed for dynamic physiological experiments.

Purpose of the Study:

  • To design and validate a device for rapid solution switching around single cardiac cells.
  • To assess the device's performance in physiological applications.
  • To enable new experimental approaches in cardiac electrophysiology.

Main Methods:

  • A device using double-barreled glass tubing (theta-tubing) and a miniature solenoid was constructed.

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  • Single cardiac cells were positioned in parallel solution streams.
  • Solenoid activation rapidly switched the solution bathing the cell within milliseconds.
  • Response times were measured using potassium-induced changes in membrane potential.

    • Main Results:

    • The device achieved bulk solution switching within 7 ms.
    • Solution exchange at the cell membrane surface occurred in approximately 150 ms for guinea pig ventricular cells.
    • The delay was attributed to diffusion limitations, potentially within the transverse tubular system.
    • The switching speed permits solution changes during action potentials and voltage-clamp pulses.

    Conclusions:

    • The developed device enables rapid and precise control of the extracellular environment for single cardiac cells.
    • This technology facilitates dynamic electrophysiological studies, including during rapid cellular events.
    • The findings have implications for advancing research in cardiac cell physiology and pharmacology.