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In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases
Lucas Sagrillo-Fagundes1,2,3, Laetitia Laurent1,2,3, Josianne Bienvenue-Pariseault1,2,3
1INRS - Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, QC, Canada, H7V 1B7.
Lack of blood flow and aberrant levels of oxygenation in placentas are recurrent in pregnancy diseases, such as preeclampsia. These alterations generate situations of hypoxia and hypoxia/reoxygenation (H/R) and consequent oxidative stress, increased cell death, and inflammation in trophoblasts. The models used to understand the effects of hypoxia and H/R on trophoblasts require a rather big structure. This chapter describes the details of a suitable and reasonable approach with hypoxia chambers to expose human placental trophoblasts to variable conditions of oxygenation.
Lack of blood flow and aberrant levels of oxygenation in placentas are recurrent in pregnancy diseases, such as preeclampsia. These alterations generate situations of hypoxia and hypoxia/reoxygenation (H/R) and consequent oxidative stress, increased cell death, and inflammation in trophoblasts. The models used to understand the effects of hypoxia and H/R on trophoblasts require a rather big structure. This chapter describes the details of a suitable and reasonable approach with hypoxia chambers to expose human placental trophoblasts to variable conditions of oxygenation.
