Plasma NT-proBNP and Cell-Free DNA Concentrations after Prolonged Strenuous Exercise in Working Farm Dogs.

Area of Science:

• Veterinary Medicine
• Canine Physiology
• Biomarker Research

Background:

• Plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) and cell-free DNA (cfDNA) are elevated in myocardial dysfunction and various diseases.
• These biomarkers can be altered in humans following endurance exercise.

Purpose of the Study:

• To investigate the impact of prolonged strenuous exercise on NT-proBNP and cfDNA levels in working farm dogs.
• To assess changes in these biomarkers after intense physical activity.

Main Methods:

• A prospective cohort study involving six working farm dogs (Huntaways and heading dogs).
• Blood samples were collected before and after four days of strenuous work.
• Plasma NT-proBNP and cfDNA concentrations were measured using ELISA and fluorometry, respectively.

Main Results:

• Plasma NT-proBNP concentrations increased by 101% after work, but decreased by 16% daily with continued work.
• Plasma cfDNA concentrations rose by 138 ng/mL post-exercise.
• NT-proBNP levels in working dogs can surpass established reference ranges.

Conclusions:

• Exercise-induced NT-proBNP elevations in dogs may exceed normal reference limits, necessitating cautious interpretation of results.
• Plasma cfDNA concentrations also increase after exercise.
• Further research is required to establish reference ranges for cfDNA in healthy dogs.