Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Star-shaped PCL nanoparticles combining quercetin release and α-amylase surface functionalization enable AFM-quantified removal of biofilms of clinical Staphylococcus aureus isolates.

Biomaterials advances·2026
Same author

Developmental circuit instability in amyotrophic lateral sclerosis: from hyperexcitability to network collapse.

Brain : a journal of neurology·2026
Same author

An electrophysiological and proteomics roadmap for human induced glutamatergic neurons: fine-tuning of culture conditions for pathophysiological studies.

Cell death discovery·2026
Same author

Hierarchical Afferent Connectivity Drives Population-Wide Bursting Dynamics in a Computational Model of Human-Derived Excitatory Neuronal Networks.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Engineered biopolymeric hydrogels for in vitro modeling of equine sarcoid tumors in comparative oncology.

International journal of biological macromolecules·2026
Same author

Wearable Sensor-Based Gait Analysis in Benign Paroxysmal Positional Vertigo: Quantitative Assessment of Residual Dizziness Using the φ-Bonacci Framework.

Life (Basel, Switzerland)·2026

Related Experiment Video

Updated: Feb 17, 2026

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
09:47

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Published on: October 18, 2015

10.5K

Soft chitosan microbeads scaffold for 3D functional neuronal networks.

Maria Teresa Tedesco1, Donatella Di Lisa1, Paolo Massobrio1

  • 1University of Genova, Dept. of Informatics, Bioengineering, Robotics and System Engineering, Via Opera Pia 13, 16145, Genova, Italy.

Biomaterials
|December 4, 2017
PubMed
Summary

Researchers developed a novel 3D chitosan scaffold for culturing mammalian neurons, advancing brain-on-a-chip models. This biomimetic scaffold supports neuronal networks, aiding the study of brain function and connectivity.

Keywords:
3D networkChitosanMicro-electrode arrays (MEAs)MicrobeadsNeuronal culture

More Related Videos

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs
07:48

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

Published on: May 5, 2023

1.9K
Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue
06:17

Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue

Published on: October 23, 2015

13.0K

Related Experiment Videos

Last Updated: Feb 17, 2026

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
09:47

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Published on: October 18, 2015

10.5K
Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs
07:48

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

Published on: May 5, 2023

1.9K
Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue
06:17

Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue

Published on: October 23, 2015

13.0K

Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Tissue Engineering

Background:

  • 3D biomimetic in vitro neuronal networks are crucial for brain-on-a-chip models.
  • Hydrogel scaffolds are widely used for 3D cell cultures.
  • Limited research exists on biomimetic 3D neuronal cultures.

Purpose of the Study:

  • To investigate chitosan (CHI) microbeads as a 3D scaffold for primary neuronal cells.
  • To develop a biomimetic scaffold for studying neuronal (dys)functions and connectivity.
  • To create advanced brain-on-a-chip experimental models.

Main Methods:

  • Fabrication of chitosan microbeads.
  • Characterization of CHI microbeads using optical and atomic force microscopy.
  • Assessment of cell/scaffold interaction via transmission electron microscopy and immunocytochemistry (confocal microscopy).
  • Preliminary electrophysiological characterization using micro-electrode arrays.

Main Results:

  • Chitosan microbeads were successfully fabricated and characterized.
  • Demonstrated effective interaction between primary neuronal cells and the chitosan scaffold.
  • Provided preliminary electrophysiological data on neuronal network activity within the scaffold.

Conclusions:

  • Chitosan microbeads offer a promising biomimetic 3D scaffold for neuronal cell culture.
  • This approach facilitates the development of advanced brain-on-a-chip models.
  • Further research can explore the potential for studying neuronal connectivity and function.