Related Experiment Video
Updated: Feb 17, 2026

Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.
Published on: November 30, 2022
Exploring stress tolerance mechanism of evolved freshwater strain Chlorella sp. S30 under 30 g/L salt
Xuyang Li1, Yizhong Yuan2, Dujia Cheng2
1School of Life Science, Shanghai University, 99 Shangda Road, Shanghai 200444, China; Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China.
Abstract:
Enhancement of stress tolerance to high concentration of salt and CO2 is beneficial for CO2 capture by microalgae. Adaptive evolution was performed for improving the tolerance of a freshwater strain, Chlorella sp. AE10, to 30 g/L salt. A resulting strain denoted as Chlorella sp. S30 was obtained after 46 cycles (138 days). The stress tolerance mechanism was analyzed by comparative transcriptomic analysis. Although the evolved strain could tolerate 30 g/L salt, high salinity caused loss to photosynthesis, oxidative phosphorylation, fatty acid biosynthesis and tyrosine metabolism. The related genes of antioxidant enzymes, CO2 fixation, amino acid biosynthesis, central carbon metabolism and ABC transporter proteins were up-regulated. Besides the up-regulation of several genes in Calvin-Benson cycle, they were also identified in C4 photosynthetic pathway and crassulacean acid metabolism pathway. They were essential for the survival and CO2 fixation of Chlorella sp. S30 under 30 g/L salt and 10% CO2.
Related Concept Videos
Responses to Salt Stress
Factors Influencing Microbial Growth: Osmolarity
Responses to Drought and Flooding
Responses to Heat and Cold Stress
Other Stress Responses in Bacteria
Adaptations that Reduce Water Loss

