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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Fingerprint Analysis: Moving Toward Multiattribute Determination via Individual Markers.

Erica Brunelle1, Crystal Huynh1, Eden Alin1

  • 1Department of Chemistry, University at Albany, State University of New York , 1400 Washington Avenue, Albany, New York 12222, United States.

Analytical Chemistry
|December 5, 2017
PubMed
Summary

New forensic science methods can determine biological sex from a single fingerprint analyte. These assays target alanine and arginine, revolutionizing personal attribute identification from fingerprints.

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Area of Science:

  • Forensic Science
  • Biochemistry
  • Analytical Chemistry

Background:

  • Identifying personal attributes from fingerprints is crucial for law enforcement.
  • Current methods often require multiple analytes and time-consuming laboratory processes.
  • Previous research focused on assays analyzing multiple analytes.

Purpose of the Study:

  • To develop novel assays for identifying biological sex from a single fingerprint analyte.
  • To establish methods for single-analyte analysis to revolutionize forensic identification.
  • To enable the identification of multiple personal attributes from a single fingerprint.

Main Methods:

  • Development of an enzymatic assay targeting alanine using alanine transaminase (ALT), pyruvate oxidase (POx), and horseradish peroxidase (HRP).
  • Development of a chemical assay, the Sakaguchi test, targeting arginine.
  • Analysis of amino acid concentrations in male and female fingerprints.

Main Results:

  • Both the alanine-targeting enzymatic assay and the arginine-targeting Sakaguchi chemical assay accurately differentiated between male and female fingerprints.
  • Alanine is present in significantly higher concentrations than arginine in fingerprints.
  • Assay performance was consistent regardless of the average concentration of the targeted amino acid.

Conclusions:

  • Single-analyte assays can effectively determine biological sex from fingerprints.
  • Targeting individual amino acids transforms forensic science by enabling attribute correlation to specific analytes.
  • This approach allows for the potential identification of multiple attributes from a single fingerprint, reducing the need for extensive lab work.