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Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
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Related Experiment Video

Updated: Feb 17, 2026

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

Linking stem cell function and growth pattern of intestinal organoids.

Torsten Thalheim¹, Marianne Quaas², Maria Herberg¹

¹Interdisciplinary Centre for Bioinformatics, Leipzig University, Haertelstr. 16-18, 04107 Leipzig, Germany.

Developmental Biology

|December 5, 2017

Summary

Intestinal stem cells (ISCs) adapt to lab conditions by stabilizing Wnt-signaling, shifting organoid growth patterns. This cell-based model explains how stem cell regulation impacts tissue development and regeneration.

Keywords:

Computational modeling Intestinal stem cell Notch Organoid culture Wnt

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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

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Last Updated: Feb 17, 2026

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

Published on: April 7, 2023

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Published on: March 8, 2024

Area of Science:

Stem Cell Biology
Developmental Biology
Biophysics

Background:

Intestinal stem cells (ISCs) rely on niche signals for function.
ISC niche self-organization is observable in organoid cultures.
Pathway manipulation in organoids yields distinct growth phenotypes.

Purpose of the Study:

To develop an individual cell-based model of intestinal organoids.
To mechanistically explain observed organoid growth phenotypes.
To investigate Wnt- and Notch-signaling interdependencies in organoid shape and growth.

Main Methods:

Individual cell-based modeling of 3D organoid structures.
Simulation studies of expanding organoids.
Analysis of Wnt- and Notch-signaling pathway activities.

Main Results:

Simulations replicate how pathway alterations affect organoid cellular composition and shape.
Exogenous Wnt induces a transition from branched to cyst-like growth patterns.
Cyst-like patterns are linked to biomechanical changes conferring a growth advantage.

Conclusions:

ISC adaptation to in vitro conditions involves Wnt-activity stabilization.
Individual cell-based modeling bridges molecular regulation and tissue growth.
This approach aids understanding of tissue regeneration and development.