Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Tail-anchoring of Proteins in the ER Membrane

Tail-anchoring of Proteins in the ER Membrane

Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Albumin to Globulin Ratio Is an Independent Prognostic and Predictive Factor for Recurrence of Esophageal Cancer After Esophagectomy.

In vivo (Athens, Greece)·2026

Same author

Ultrasound-Guided Lumbar Nerve Root Hydrodissection: Fluoroscopy-Assisted Evaluation of Injectate Distribution and Feasibility.

Spine surgery and related research·2026

Same author

Clinical course after cardiac resynchronization therapy in transthyretin amyloid cardiomyopathy receiving disease-modifying therapy.

Heart and vessels·2026

Same author

Kynurenic acid mediates epicardial fat-induced lymphatic metabolic dysfunction in atrial fibrillation.

Nature communications·2026

Same author

Correction to "Electron Transfer Enhanced by a Minimal Energetic Driving Force at the Organic-Semiconductor Interface".

Angewandte Chemie (International ed. in English)·2026

Same author

Author Correction: Blue organic light-emitting diode with a turn-on voltage of 1.47V.

Nature communications·2026

Same journal

Differential cardiac microRNA expression in anoxic Trachemys scripta elegans turtles.

Biochimie·2026

Same journal

Renal failure-driven luminal ammonia production impairs gut barrier function in CKD.

Biochimie·2026

Same journal

Conditional Knockout of Indoleamine 2, 3-Dioxygenase-1 in Osteoprogenitor Cells in Mice Results in Sex-dependent Differences in Bone Mass.

Biochimie·2026

Same journal

Sedentariness disrupts, while exercise restores, thermogenic and metabolic plasticity in inguinal adipose tissue of mice.

Biochimie·2026

Same journal

Chrononutrition as a modulator of retinal metabolic resilience: A translational framework linking circadian biology to ocular disease.

Biochimie·2026

Same journal

Heterologous expression, purification, and biophysical characterisation of the cobalt-dependent nitrile hydratase from Rhodococcus rhodochrous ATCC BAA-870.

Biochimie·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 17, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Aptamers targeting cell surface proteins.

Masaki Takahashi¹

¹Division of RNA Medical Science, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo, Japan.

|December 5, 2017

Summary

This summary is machine-generated.

Nucleic acid aptamers offer a powerful method for discovering high-affinity binders to cell surface proteins. Systematic Evolution of Ligands by Exponential Enrichment (SELEX) enables aptamer generation against complex targets like whole cells.

Keywords:

Aptamer Cell surface protein Nucleic acid SELEX

More Related Videos

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Published on: June 23, 2011

Related Experiment Videos

Last Updated: Feb 17, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Published on: June 23, 2011

Area of Science:

Biotechnology
Molecular Biology
Biochemistry

Background:

High-affinity binders for cell surface proteins are crucial for biosciences.
Existing methods like antibodies have limitations in targeting all cell surface proteins.
Nucleic acid aptamers present a promising alternative due to their structural versatility and specificity.

Purpose of the Study:

To review and update Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technologies.
To highlight the application of SELEX in generating aptamers targeting membrane proteins.
To underscore the advantages of SELEX for cell surface protein binder development.

Main Methods:

SELEX is an iterative selection-amplification process for isolating aptamers.
SELEX utilizes large combinatorial libraries to identify high-affinity binders.
This method allows selection against complex targets, including whole living cells.

Main Results:

SELEX enables the generation of aptamers with high affinity and specificity for cell surface proteins.
The technology can be applied to targets without prior knowledge of their expression profiles.
SELEX is effective for producing aptamers that bind to cell surface proteins in their native structure.

Conclusions:

SELEX is a validated and feasible technology for aptamer generation against cell surface proteins.
Aptamers generated via SELEX are valuable tools for both basic and applied biosciences.
This review consolidates key SELEX strategies for targeting membrane proteins.