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Author Spotlight: Enhancing Dental Pulp Research with Improved Mouse Models
Published on: October 27, 2023
[Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue]
Zhai Yue1, Jian-Ying Huang, Park Hyun
1Department of General Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine；Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011,China.
Purpose:
To investigate the expression of human β-defensin(HBD) in human dental pulp tissue and to explore the regulation of HBD in pulp inflammation and the relationship among HBD family members.
Methods:
The gene expression of HBD in human dental pulp tissue was assessed in NCBI GEO profiles and was verified by RT-PCR. Human dental pulp cells were stimulated with TNF-α, IL-1α, IL-1β and IL-6 in different combinations and the expression of HBD2 was analyzed by qPCR. Human dental pulp cells were pretreated with HBD110 and then stimulated with LPS and the expression of TNF-α,IL-1α and HBD2 were analyzed by qPCR. GraphPad Prism 5.01 was used to analyze the results of the experimental and the control groups.
Results:
27 HBDs were found to express in human dental pulp tissue in NCBI GEO Profiles. The joint overexpression of TNF-α, IL-1α, IL-1β and IL-6 increased the expression of HBD2; HBD110 increased the expression of HBD2 by increasing the expression of TNF-α and IL-1α.
Conclusions:
Many other HBDs have positive expression in human dental pulp issue besides of HBD1, HBD2, HBD3, HBD4 and the inflammation factors and other HBDs can regulate the expression of HBD2 in dental pulp.
