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Updated: Feb 17, 2026
Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
Published on: June 16, 2023
Long non-coding RNA ANRIL promotes the proliferation, migration and invasion of human osteosarcoma cells
Shuo Cheng1, Tao Huang1, Pengcheng Li1
1Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Abstract:
The long antisense non-coding RNA ANRIL is 3.8 kb in length and serves an important role in the tumorigenesis and progression of a number of malignancies. However, its function in human osteosarcoma remains unknown. The present study investigated the role of ANRIL in human osteosarcoma cell lines and clinical tumor samples. The expression of ANRIL was analyzed in 19 osteosarcoma and paired adjacent non-cancerous tissues using reverse transcription-quantitative polymerase chain reaction. Knockdown of ANRIL expression using lentivirus-mediated small interfering RNA was performed to investigate the role of ANRIL in tumor proliferation and metastasis using MTT, colony formation and transwell assays. The results demonstrated that ANRIL expression was upregulated in osteosarcoma tissues when compared with ANCT samples. In addition, knockdown of ANRIL expression in vitro reduced cell proliferation and invasion. These results indicated that ANRIL is upregulated in osteosarcoma tissues, and may promote the proliferation and metastasis of osteosarcoma cells. Therefore, ANRIL may serve as a novel biomarker and target of novel therapies for the treatment of patients with osteosarcoma.
Insights
The long antisense non-coding RNA (ANRIL) is upregulated in osteosarcoma, promoting tumor cell proliferation and metastasis. ANRIL may be a potential therapeutic target for osteosarcoma treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The long antisense non-coding RNA (ANRIL) is implicated in various cancers.
- The role of ANRIL in human osteosarcoma pathogenesis is currently unknown.
Purpose of the Study:
- To investigate the expression and function of ANRIL in human osteosarcoma.
- To determine if ANRIL could serve as a biomarker or therapeutic target for osteosarcoma.
Main Methods:
- ANRIL expression was quantified in osteosarcoma tissues and adjacent non-cancerous tissues using reverse transcription-quantitative polymerase chain reaction.
- Lentivirus-mediated small interfering RNA was used to knockdown ANRIL expression in osteosarcoma cell lines.
- Cell proliferation and invasion were assessed using MTT, colony formation, and transwell assays.
Main Results:
- ANRIL expression was significantly upregulated in osteosarcoma tissues compared to adjacent non-cancerous tissues.
- Knockdown of ANRIL expression in vitro led to reduced osteosarcoma cell proliferation and invasion.
- These findings suggest ANRIL promotes osteosarcoma cell growth and metastasis.
Conclusions:
- ANRIL is upregulated in human osteosarcoma and promotes tumor cell proliferation and metastasis.
- ANRIL represents a potential novel biomarker for osteosarcoma diagnosis.
- Targeting ANRIL may offer a new therapeutic strategy for osteosarcoma treatment.
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