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Updated: Feb 17, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
Published on: February 25, 2016
Endothelial nitric oxide synthase gene polymorphisms in patients with slow coronary flow
Nurzen Sezgin1, Abdullah Tekin2, Fatma Belgin Atac3
1Department of Biochemistry, Acıbadem University School of Medicine, İstanbul, Turkey.
Background And Aims:
The aim of this study was to explore potential associations of the intron 4 variable number of tandem repeats (VNTR) and E298A polymorphisms of the endothelial nitric oxide synthase (eNOS) gene with slow coronary flow (SCF). The association between plasma nitrate and nitrite (NO ) concentrations and eNOS gene polymorphisms was also assessed.
Materials And Methods:
The intron 4 VNTR and E298A polymorphisms of the eNOS gene were evaluated in the isolated DNA blood samples obtained from the SCF patient group (n = 30) and healthy group consisted of age- and sex-matched controls (n = 61).
Results:
Plasma NO level was significantly lower in patients with SCF than in controls. In addition, patients with SCF have significantly lower nitric oxide levels than control subjects within each genotype variants. The allele and genotyped frequencies of the eNOS intron 4 VNTR and E298A polymorphisms were similar between patients with SCF and the controls. Plasma NO concentrations with respect to the relevant genotypes were found insignificant.
Discussion And Conclusion:
Plasma NO is lower in patients with SCF than in healthy subjects. Our findings may suggest the lack of association between intron 4 VNTR and E298A polymorphisms of the eNOS gene and SCF.
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