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Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Laser scanning laser diode photoacoustic microscopy system.

Mohsen Erfanzadeh¹, Patrick D Kumavor¹, Quing Zhu²

¹Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.

|December 5, 2017

Summary

A new, low-cost laser scanning photoacoustic microscopy system offers fast imaging speeds of 370 A-lines/sec. This advancement improves the clinical potential of photoacoustic imaging (PAI) for various biological samples.

Keywords:

Biological imaging Diode lasers Low-cost sources Medical imaging Photoacoustic imaging

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Published on: May 30, 2019

Area of Science:

Biomedical Optics
Medical Imaging
Photoacoustics

Background:

Advancements in low-cost and high-speed photoacoustic microscopy (PAM) are crucial for expanding the clinical applications of photoacoustic imaging (PAI).
Existing PAI systems can be expensive and slow, limiting their widespread clinical adoption.
Developing accessible and rapid PAM systems is a key research objective.

Purpose of the Study:

To present a novel, low-cost, laser scanning laser diode-based photoacoustic microscopy system.
To evaluate the performance characteristics, including resolution and imaging speed, of the developed PAM system.
To demonstrate the system's feasibility for imaging biological tissues.

Main Methods:

Utilized a 905 nm, 325 W pulsed laser diode (50 ns pulsewidth) as the light source.
Employed aspheric and cylindrical lenses for beam collimation and an aspheric lens for focusing.
Incorporated galvanometer scanning mirrors for beam steering and measured lateral resolution (~21 μm) via edge spread function estimation.

Main Results:

Achieved a lateral resolution of approximately 21 μm without data averaging.
Demonstrated a high imaging speed of approximately 370 A-lines per second.
Successfully acquired photoacoustic microscopy images of human hairs, ex vivo mouse ear, and ex vivo porcine ovary.

Conclusions:

The developed laser scanning laser diode-based photoacoustic microscopy system is a low-cost and fast solution.
The system exhibits promising performance for biomedical imaging applications.
The demonstrated feasibility suggests potential for enhanced clinical applicability of photoacoustic imaging.