Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adaptable centriole biogenesis via the intrinsically disordered protein ALMS1.

Nature communications·2026
Same author

DNER drives glycolytic reprogramming in renal cell carcinoma by activating the JAK2/STAT3 signaling pathway.

Frontiers in immunology·2026
Same author

High-fold Homogeneous Expansion Microscopy Reveals Ultrastructural Centrioles.

ACS nano·2026
Same author

JMJD6-mediated epigenetic silencing of innate immunity promotes pseudorabies virus replication.

Journal of virology·2026
Same author

Case Report: Gallbladder perforation after endoscopic retrograde cholangiopancreatography-a rare complication.

Frontiers in medicine·2026
Same author

Characterizing transcriptomic signatures and identifying hub differentially expressed genes in resistant hypertension.

Yi chuan = Hereditas·2026

Related Experiment Video

Updated: Feb 17, 2026

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB
08:10

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

Published on: August 21, 2018

11.6K

Quantitative Serum Proteomic Analysis of Essential Hypertension Using iTRAQ Technique.

Jing-Wen Xu1,2,3, Yun-Lun Li1,2, Shi-Jun Zhang1

  • 1Shandong University of Traditional Chinese Medicine, 4655 Daxue Road, Changqing District, Jinan, Shandong Province, China.

Biomed Research International
|December 5, 2017
PubMed
Summary

This study identified four key serum proteins involved in essential hypertension (EH) pathophysiology. Their altered levels may help predict EH and offer new therapeutic targets for this common cardiovascular condition.

More Related Videos

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K
Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI
08:57

Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI

Published on: August 18, 2017

8.4K

Related Experiment Videos

Last Updated: Feb 17, 2026

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB
08:10

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

Published on: August 21, 2018

11.6K
Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K
Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI
08:57

Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI

Published on: August 18, 2017

8.4K

Area of Science:

  • Biochemistry
  • Proteomics
  • Cardiovascular Research

Background:

  • Essential hypertension (EH) is a significant risk factor for severe diseases, necessitating a deeper understanding of its underlying mechanisms.
  • Identifying specific serum proteins and their interactions could reveal novel therapeutic targets for EH.
  • Current knowledge of EH pathophysiology requires expansion to include proteomic insights.

Purpose of the Study:

  • To screen for unique serum proteins in patients with essential hypertension (EH) compared to healthy controls.
  • To investigate the interactions between identified proteins and their potential roles in EH.
  • To provide new therapeutic targets and elucidate the comprehensive pathophysiological mechanisms of EH.

Main Methods:

  • Serum samples from 47 EH patients and 47 healthy controls were analyzed using quantitative proteomics (isobaric tags for relative and absolute quantitation coupled with 2D-LC-ESI-MS/MS).
  • Gene Ontology (GO) and KEGG pathway analyses were performed to understand protein functions and interactions.
  • Candidate proteins were validated using Enzyme-Linked Immunosorbent Assay (ELISA) and Western blot.

Main Results:

  • A total of 404 proteins were identified, with 30 upregulated and 81 downregulated in EH patients compared to controls (p < 0.05).
  • Four proteins—cathepsin G, transforming growth factor beta-1, hyaluronidase-1, and kininogen-1—were identified as jointly involved in the renin-angiotensin-aldosterone system and kallikrein-kinin system.
  • ELISA and Western blot confirmed the differential expression profiles of these four candidate proteins.

Conclusions:

  • The identified serum proteins, cathepsin G, transforming growth factor beta-1, hyaluronidase-1, and kininogen-1, play a role in the pathophysiology of essential hypertension.
  • Variations in the concentrations of these four proteins can potentially predict EH occurrence and elucidate its mechanisms.
  • These findings offer promising avenues for developing novel therapeutic strategies for essential hypertension.