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A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
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A broad-host range dual-fluorescence reporter system for gene expression analysis in Gram-negative bacteria
Rosanna C Hennessy1, Line Christiansen1, Stefan Olsson2
1Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
Journal of Microbiological Methods
|December 6, 2017
Abstract:
Fluorescence-based reporter systems are valuable tools for studying gene expression dynamics in living cells. Here we describe a dual-fluorescence reporter system carrying the red fluorescent marker mCherry and the blue fluorescent protein EBFP2 enabling the simultaneous analysis of two promoters in broad-host range autofluorescent Gram-negative bacteria.

