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Updated: Feb 17, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
RNA binding protein Lin28A promotes osteocarcinoma cells progression by associating with the long noncoding RNA
Zhen Wang1, Jinhui Pang1, Bin Ji1
1Department of Orthopedics, Shanghai Putuo District Central Hospital, Shanghai, 200062, China.
Objectives:
To explore the effects of Lin28A on progression of osteocarcinoma (OS) cells.
Results:
Lin28A mRNA and protein expressions were significantly increased in OS tissues compared with that in normal adjacent tissues. Expressions of Lin28A and long noncoding RNA MALAT1 were positively correlated. Patients with higher Lin28A expression had shorter overall survival. Moreover, Lin28A knockdown inhibited OS cells proliferation, migration, invasion and promoted cell apoptosis; Lin28A was found to harbor binding sites on MALAT1 sequences and associated with MALAT1, and increased MALAT1 stability and expression. Notably, the inhibition of Lin28A knockdown was attenuated or even reversed by MALAT1 overexpression.
Conclusions:
RNA binding protein Lin28A could facilitate OS cells progression by associating with the long noncoding RNA MALAT1.
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