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[Application of Multiple Displacement Amplification in Samples with Inhibitors]
1Department of Forensic Serology, Faculty of Forensic Medicine, China Medical University, Shenyang 110001, China.
Fa Yi Xue Za Zhi
|December 6, 2017
Summary
Multiple displacement amplification (MDA) effectively overcomes DNA inhibition from hemoglobin and humic acid, outperforming magnetic beads methods (MBM) in forensic applications.
Area of Science:
- Forensic Science
- Molecular Biology
- Genetics
Background:
- Forensic DNA analysis often encounters inhibitors like hemoglobin and humic acid, which can impede amplification.
- Effective sample purification is crucial for successful DNA profiling in forensic investigations.
Purpose of the Study:
- To evaluate the inhibitor resistance of Multiple Displacement Amplification (MDA).
- To compare the efficacy of MDA with Magnetic Beads Method (MBM) for DNA purification in the presence of inhibitors.
- To determine the forensic applicability of MDA.
Main Methods:
- DNA samples were spiked with varying concentrations of hemoglobin and humic acid.
- Samples were processed using MDA, MBM, or served as controls.
- The D3S1358 locus was amplified and analyzed using capillary electrophoresis.
Main Results:
- Control samples failed amplification at low inhibitor concentrations (hemoglobin > 1 ng/μL, humic acid > 0.1 ng/μL).
- MBM failed amplification at higher inhibitor concentrations (hemoglobin > 100 ng/μL, humic acid > 1 ng/μL).
- MDA successfully amplified DNA across all tested inhibitor concentrations without adverse effects.
Conclusions:
- MDA demonstrates superior resistance to hemoglobin and humic acid inhibition compared to MBM.
- MDA offers significant advantages for forensic DNA analysis, particularly with challenging samples.
- MDA holds considerable value for practical forensic applications.
