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Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
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Multiplex three-dimensional optical mapping of tumor immune microenvironment.

Steve Seung-Young Lee1,2, Vytautas P Bindokas3, Stephen J Kron4,5

  • 1Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, USA.

Scientific Reports
|December 7, 2017
PubMed
Summary

Transparent Tissue Tomography (T3) enables rapid, high-resolution 3D imaging of tumors. This method reveals complex tumor microenvironments and biomarker distributions, aiding in understanding cancer heterogeneity.

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Area of Science:

  • Biomedical Imaging
  • Oncology
  • Immunohistochemistry

Background:

  • Advancements in optical clearing and microscopy enable high-resolution 3D tissue visualization.
  • Current methods face limitations for oncology applications, necessitating improved techniques for tumor analysis.

Purpose of the Study:

  • To introduce Transparent Tissue Tomography (T3) for rapid, 3D, multiplexed immunofluorescent tumor imaging.
  • To demonstrate T3's utility in mapping tumor microenvironments and biomarkers at high resolution.

Main Methods:

  • Tumors are sectioned into macrosections for staining, clearing, and confocal imaging.
  • Image registration and fusion create high-resolution 3D maps of tumor components.
  • Automated image analysis quantifies biomarker distribution and spatial correlations.

Main Results:

  • T3 revealed heterogeneous programmed death-ligand 1 (PD-L1) distribution in mouse mammary tumors.
  • High PD-L1 expression was observed in peripheral tumor cells and core vascular endothelium (CD31+).
  • Strong spatial correlation between immune cells (CD45+) and PD-L1 expression was identified.

Conclusions:

  • T3 provides rapid, high-resolution 3D mapping of the tumor immune microenvironment.
  • This tomographic approach is a valuable new tool for examining tumor heterogeneity in oncology.