Related Experiment Video

Updated: Feb 17, 2026

Author Spotlight: Advancing Thrombolytic Testing by Integrating Flow Dynamics in In Vitro Models
06:16

Author Spotlight: Advancing Thrombolytic Testing by Integrating Flow Dynamics in In Vitro Models

Published on: April 19, 2024

1.9K

Zinc chelation promotes streptokinase-induced thrombolysis in vitro

Zihui Wang1,2, Xinge Yu1,2, Yang V Li2

  • 1Department of Biological Sciences, Ohio UniversityAthens Ohio, USA.

International Journal of Physiology, Pathophysiology and Pharmacology
|December 7, 2017
PubMed

Insights

Zinc inhibits streptokinase-induced thrombolysis, while zinc chelation with Ca-EDTA enhances its effectiveness. This finding may reduce adverse effects of streptokinase therapy for cardiovascular disorders.

Area of Science:

  • Biochemistry
  • Cardiovascular Research
  • Pharmacology

Background:

  • Cardiovascular disorders, often caused by blood clots, are a leading cause of mortality and disability.
  • Thrombolytic agents like streptokinase (SK) are crucial for dissolving clots and restoring blood flow, but high doses raise safety concerns.
  • Understanding factors influencing streptokinase efficacy is vital for improving treatment outcomes and patient safety.

Purpose of the Study:

  • To investigate the effect of zinc on streptokinase-induced thrombolysis in vitro.
  • To explore the potential of zinc chelation as a strategy to enhance streptokinase's thrombolytic activity.
  • To assess the clinical implications of modulating zinc levels during thrombolytic therapy.

Main Methods:

  • Blood clots were formed from mice whole blood in vitro by incubation with calcium.
  • Streptokinase was used to induce thrombolysis, with effects measured spectrophotometrically.
  • The impact of co-applied zinc and the zinc chelator Ca-EDTA on streptokinase activity was evaluated.

Main Results:

  • Zinc significantly inhibited streptokinase-induced thrombolysis in a dose-dependent manner.
  • The zinc chelator Ca-EDTA markedly potentiated the thrombolytic effect of streptokinase.
  • These findings indicate that zinc interferes with and chelation enhances streptokinase's action.

Conclusions:

  • Zinc chelation improves the efficiency of streptokinase in promoting thrombolysis.
  • Modulating zinc levels presents a potential strategy to enhance the effectiveness of streptokinase therapy.
  • This research may lead to reduced adverse effects and improved outcomes in treating thrombotic disorders.

Keywords:
Streptokinaseclotthrombolysiszinczinc chelation

More Related Videos

Ferric Chloride-induced Murine Thrombosis Models10:37

Ferric Chloride-induced Murine Thrombosis Models

Published on: September 5, 2016

23.2K
Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel07:26

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

Published on: June 29, 2015

18.8K

Related Concept Videos

Venous Thrombosis III: Interprofessional Care01:29

Venous Thrombosis III: Interprofessional Care

Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...
388
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
2.0K
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
9.2K
Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants01:18

Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants

Oral anticoagulants are vital tools in preventing and treating blood clotting disorders. This diverse class of medications can be categorized as vitamin K antagonists, exemplified by warfarin, and direct thrombin inhibitors (DTIs), such as dabigatran, as well as factor Xa inhibitors, including rivaroxaban.
Warfarin, a prominent vitamin K antagonist family member, exerts its effect by inhibiting the enzyme VKORC1 (vitamin K epoxide reductase complex 1). By hindering this enzyme, warfarin...
2.5K
EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
1.4K