Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

3.8K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.8K
Proteomics01:33

Proteomics

9.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic profile of bone in patients with acromegaly: new insights from a pilot exploratory study.

Journal of endocrinological investigation·2026
Same author

Human Chorionic Gonadotropin (hCG)-Induced Remodeling of the Granulosa Cell Exosomal Proteome: Implications for Follicular Communication.

Cells·2026
Same author

Personalized automatic management of tracheal cuff pressure and subglottic secretions drainage to prevent pneumonia in critically ill intubated patients. The MICROINHALO multicenter randomized controlled trial.

Intensive care medicine·2026
Same author

Ultrasound-guided vs conventional venous puncture for atrial fibrillation ablation: the ULYSSES trial.

European heart journal·2026
Same author

Polygenic Risk Scores disclosure for cardiovascular prevention: Protocol of the Personalized HeartCare (PHC) trial.

PloS one·2026
Same author

Seminal Redox Improvement and Sperm Proteome Remodeling After Deprox-HP Nutraceutical Supplementation in Male Accessory Gland Inflammation: A Pilot Study.

International journal of molecular sciences·2026
Same journal

Core-shell structured nanomaterials in dual-modal magnetic resonance imaging guided antitumor effect via combined treatment.

Frontiers in chemistry·2026
Same journal

Photo-responsive nanozymes: from photocatalytic mechanisms to precision therapy.

Frontiers in chemistry·2026
Same journal

From theoretical energy to practical utilization: interfacial stability, transport kinetics, and cell-level design in high-energy lithium-metal batteries.

Frontiers in chemistry·2026
Same journal

Zinc-vacancy defects in ZnO nanorods induced visible-light activity of photoelectrochemical glucose sensing: experimental and DFT+U analysis.

Frontiers in chemistry·2026
Same journal

Integrating multi-isotope calibration and infrared-assisted digestion for robust and sustainable multielemental determination in agroalimentary matrices by ICP-MS.

Frontiers in chemistry·2026
Same journal

Simultaneous phosphorus recovery as vivianite crystallization and hydrogen generation from acidified oil wastewater by Fe-C micro-electrolysis.

Frontiers in chemistry·2026
See all related articles

Related Experiment Video

Updated: Feb 17, 2026

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

1.2K

A Perspective on Extracellular Vesicles Proteomics.

Livia Rosa-Fernandes1, Victória Bombarda Rocha1, Victor Corasolla Carregari2

  • 1GlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Frontiers in Chemistry
|December 7, 2017
PubMed
Summary
This summary is machine-generated.

Extracellular vesicles (EVs) are key messengers in health and disease. This review details advanced proteomic and bioinformatics methods for analyzing EV proteins and their functions.

Keywords:
bottom-upcrosslinkingexosomesextracellular vesiclesmass spectrometrypost-translational modificationproteomicstop-down

More Related Videos

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.7K
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

5.8K

Related Experiment Videos

Last Updated: Feb 17, 2026

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

1.2K
Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.7K
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

5.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Extracellular vesicles (EVs) are increasingly recognized for their roles in intercellular communication.
  • Their molecular cargo is crucial for understanding homeostasis and disease.
  • Proteomic analysis of EVs is essential for deciphering their functions.

Purpose of the Study:

  • To review state-of-the-art proteomic technologies for EV protein characterization.
  • To highlight advancements in mass spectrometry for analyzing EV proteins, post-translational modifications (PTMs), and interactions.
  • To discuss the application of bioinformatics in understanding the EV proteome and its pathophysiological relevance.

Main Methods:

  • Mass spectrometry-based proteomics for protein identification and quantification.
  • Analysis of post-translational modifications (PTMs) and protein interactions.
  • Bioinformatics approaches for interpreting EV proteomic data.

Main Results:

  • Proteomic technologies, particularly mass spectrometry, have significantly advanced the detailed characterization of EV proteins.
  • These methods enable the analysis of limited EV amounts from various biological sources.
  • Bioinformatics tools are crucial for creating molecular fingerprints of EVs.

Conclusions:

  • Advanced proteomic and bioinformatics strategies are vital for comprehensive EV characterization.
  • Understanding the EV proteome deepens insights into their roles in physiological and pathological processes.
  • Future research should focus on overcoming challenges in EV proteome analysis for better diagnostic and therapeutic applications.