Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patterns of osseous healing and remodelling after intraoral vertical ramus osteotomy.

International journal of oral and maxillofacial surgery·2026
Same author

PREVALENCE AND ASSOCIATED RISK FACTORS OF NOCTURNAL ENURESIS AMONG CHILDREN AGED 5-18 YEARS IN ALBAHA REGION, SAUDI ARABIA.

Georgian medical news·2025
Same author

Virus-Specific T-Cell Therapy for Prophylaxis and Treatment of Cytomegalovirus Infections After Transplantation: a Scoping Review.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2025
Same author

PREVALENCE, PATTERN, RISK FACTORS, AND MANAGEMENT OF ABDOMINAL AND INGUINAL HERNIAS IN KING FAHAD HOSPITAL AT AL-BAHA CITY, SAUDI ARABIA 2024.

Georgian medical news·2025
Same author

EFFECT OF ENLARGED ADENOIDS AND TONSILS ON BLOOD OXYGEN SATURATION IN AL BAHA, SAUDI ARABIA.

Georgian medical news·2024
Same author

Association between COVID-19 exposure and autonomic nervous system dysfunction in apparently healthy adults: an observational study.

European review for medical and pharmacological sciences·2024
Same journal

Unsupervised clustering FLIM-phasor from multifunctionalized nanoparticles in living cancer cells.

Methods and applications in fluorescence·2026
Same journal

Resonance acoustic mixing-enabled non-covalent loading of Cy7 for enhanced solubility, stability, and fluorescent imaging performance.

Methods and applications in fluorescence·2026
Same journal

Substituent position-dependent photophysics in fluorinated porphycenes.

Methods and applications in fluorescence·2026
Same journal

Selective luminescence enhancement of lanthanide complexes using diffraction gratings.

Methods and applications in fluorescence·2026
Same journal

Fluorescence-based trace detection of explosives: current state and perspective for ultrasensitive portable technique via plasmonic enhancement.

Methods and applications in fluorescence·2026
Same journal

Compensating for photon counting losses in a TCSPC SPAD array enables quantitative time-resolved fluorescence anisotropy imaging.

Methods and applications in fluorescence·2026
See all related articles

Related Experiment Video

Updated: Feb 17, 2026

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
08:25

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

4.1K

Detecting beta-amyloid aggregation from time-resolved emission spectra.

A Alghamdi1, V Vyshemirsky2, D J S Birch1,3

  • 1Photophysics Group, Centre for Molecular Nanometrology, Department of Physics, Scottish Universities Physics Alliance, University of Strathclyde, 107 Rottenrow, Glasgow G4 0NG, United Kingdom.

Methods and Applications in Fluorescence
|December 7, 2017
PubMed
Summary
This summary is machine-generated.

Detecting beta-amyloid oligomers early is crucial for Alzheimer's disease diagnosis. Time-resolved emission spectra reveal structural changes in beta-amyloid during aggregation, aiding early detection.

More Related Videos

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
10:04

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

14.1K
Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging
09:31

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Published on: March 27, 2020

7.7K

Related Experiment Videos

Last Updated: Feb 17, 2026

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
08:25

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

4.1K
Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
10:04

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

14.1K
Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging
09:31

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Published on: March 27, 2020

7.7K

Area of Science:

  • Biochemistry
  • Neuroscience
  • Biophysics

Background:

  • Alzheimer's disease is linked to beta-amyloid aggregation.
  • Early detection of beta-amyloid oligomers is vital for therapeutic development.
  • Previous studies used fluorescence intensity decay to monitor early aggregation stages.

Purpose of the Study:

  • To present a complementary approach using time-resolved emission spectra (TRES) for detecting beta-amyloid oligomers.
  • To reveal structural changes in beta-amyloid on the nanosecond timescale.
  • To correlate spectral shifts with the extent of beta-amyloid aggregation.

Main Methods:

  • Utilizing time-resolved emission spectra (TRES) of tyrosine in beta-amyloid.
  • Excitation at 279 nm with fluorescence detection between 240-450 nm.
  • Analyzing dielectric relaxation dynamics of beta-amyloid monomers and oligomers.

Main Results:

  • TRES successfully resolved fluorescent species, distinguishing monomers from oligomers.
  • Beta-amyloid monomers exhibited rapid dielectric relaxation.
  • Oligomers showed significant spectral shifts due to dielectric relaxation, decreasing with increasing oligomer size.

Conclusions:

  • TRES is advantageous over steady-state spectra for studying nanosecond structural dynamics.
  • Observed spectral shifts correlate with beta-amyloid aggregation states.
  • This method offers a sensitive approach for early-stage Alzheimer's disease research.