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Updated: Feb 17, 2026
Isolation, Processing and Analysis of Murine Gingival Cells
Published on: July 2, 2013
Cell Derived Microparticles in Gingival Crevicular Fluid from Periodontitis Patients with Type 2 Diabetes
Carlos Marcelo Figueredo1, Ronaldo Lira1, Manuela Rubim Sete1
1Department of Periodontology, School of Dentistry, UERJ - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Abstract:
Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. However, its role in the periodontal disease pathogenesis remains unclear. Therefore, we aimed to detect the presence neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival crevicular fluid from individuals having periodontitis aggravated by type 2 diabetes. Twelve patients (56.2 ±7.2 yrs) with severe form of chronic periodontitis aggravated by type 2 diabetes were included. Clinical and metabolic data were gathered. Gingival crevicular fluid was collected using filter strips from deep and shallow sites. MPs were detected by flow cytometry according to their size (< 1 µm) and the expression of surface markers (CD66b for neutrophil-derived MPs and CD41b for platelet-derived MPs). All samples were positive for the antibodies. Median levels of CD66b+ MPs and CD41b+ MPs were, respectively, 3,677.0 (2,553.2 - 9,059.8) MP/µL and 520.7 (432.9 - 766.1) MP/µL in deep sites. In shallow sites, the corresponding values were 2,644.9 (1,451.5 - 3,858.9) MP/µL and 371.2 (287.2 - 692.7) MP/µL. There was no significant difference between deep and shallow sites (p>0.05). In conclusion, this study reported the presence of neutrophil and platelet derived microparticles in gingival crevicular fluid from individuals having severe periodontitis and type 2 diabetes.
Insights
Neutrophil and platelet microparticles are present in gingival crevicular fluid of patients with severe periodontitis and type 2 diabetes. This finding may offer new insights into periodontal disease pathogenesis and inflammation.
Area of Science:
- Periodontology
- Immunology
- Endocrinology
Background:
- Cell-derived microparticles (MPs) are implicated in inflammation.
- The specific role of MPs in periodontal disease pathogenesis, especially when aggravated by type 2 diabetes, is not well understood.
Purpose of the Study:
- To detect neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival crevicular fluid.
- To investigate the presence of these microparticles in individuals with severe periodontitis and type 2 diabetes.
Main Methods:
- Gingival crevicular fluid was collected from deep and shallow sites in twelve patients with severe periodontitis and type 2 diabetes.
- Microparticles (< 1 µm) were quantified using flow cytometry.
- Surface markers CD66b (neutrophil-derived) and CD41b (platelet-derived) were used for identification.
Main Results:
- All samples tested positive for neutrophil-derived MPs (CD66b+) and platelet-derived MPs (CD41b+).
- Median levels of CD66b+ MPs were 3,677.0 MP/µL in deep sites and 2,644.9 MP/µL in shallow sites.
- Median levels of CD41b+ MPs were 520.7 MP/µL in deep sites and 371.2 MP/µL in shallow sites.
- No significant difference in MP levels was observed between deep and shallow sites (p>0.05).
Conclusions:
- Neutrophil and platelet-derived microparticles are present in the gingival crevicular fluid of individuals with severe periodontitis and type 2 diabetes.
- These findings contribute to understanding the role of microparticles in the inflammatory processes associated with this condition.
