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Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) promotes lung tumorigenesis via attenuating p53

Wei Zhao1,2, Dan Lu3, Liang Liu3

  • 1Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Key Laboratory of Medical Immunology, Ministry of Health (Peking University), Beijing, 100191, P.R. China.

Oncotarget
|December 8, 2017
PubMed

Insights

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) promotes lung cancer by reducing p53 stability. This study reveals IGF2BP3

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) is highly expressed in embryonic tissues and cancers.
  • IGF2BP3 is a potential diagnostic marker for lung cancer, but its function in lung tumorigenesis is unclear.

Purpose of the Study:

  • To investigate the functional role of IGF2BP3 in lung cancer development.
  • To elucidate the molecular mechanism by which IGF2BP3 influences lung tumorigenesis.

Main Methods:

  • Quantitative analysis of IGF2BP3 mRNA and protein levels in lung cancer tissues versus normal tissues.
  • In vitro and in vivo experiments assessing the effects of IGF2BP3 overexpression and knockdown on lung cancer cell behavior.
  • Investigation of the interaction between IGF2BP3 and USP10, and its impact on p53 protein stability.

Main Results:

  • IGF2BP3 expression is significantly upregulated in lung cancer tissues at both mRNA and protein levels.
  • Overexpression of IGF2BP3 enhances lung cancer cell proliferation, migration, and invasion, while knockdown inhibits these processes.
  • IGF2BP3 interacts with USP10, attenuating p53 protein stability, leading to increased p53 half-life and protein levels upon IGF2BP3 silencing, and inducing G0/G1 cell cycle arrest.

Conclusions:

  • IGF2BP3 promotes lung tumorigenesis by inhibiting p53 protein stability through interaction with USP10.
  • Targeting IGF2BP3 may represent a potential therapeutic strategy for lung cancer.
Keywords:
IGF2BP3USP10lung cancerp53proliferation

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