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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Circulating MicroRNA Profiles Differ between Hyperglycemia and Euglycemia in Coronary Heart Disease Patients
Yunyao Jiang1,2,3, Nan Liu1,2,4, Bingjie Xue1,2,3
1Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Insights
This study investigated circulating microRNAs (miRNAs) in patients with coronary heart disease (CHD), finding specific miRNA patterns in those with hyperglycemia. These findings may help differentiate CHD subtypes and identify key miRNAs like hsa-let-7c-5p and hsa-miR-24-3p.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Genomics
Background:
- Coronary heart disease (CHD) is a leading cause of mortality and morbidity worldwide.
- Hyperglycemia is a known risk factor for cardiovascular disease.
- MicroRNAs (miRNAs) in peripheral blood are potential biomarkers for CHD discrimination.
Purpose of the Study:
- To investigate the biological characteristics of coronary heart disease with hyperglycemia (HCHD) versus coronary heart disease with euglycemia (ECHD).
- To identify differentially expressed circulating miRNAs in HCHD and ECHD patients.
- To explore the functional roles and regulatory networks of these miRNAs.
Main Methods:
- Microarray analysis of circulating miRNAs from 26 HCHD patients and 42 ECHD patients.
- Gene Ontology (GO) analysis using DAVID for functional annotation of miRNA targets.
- Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
- Construction of miRNA-gene regulatory networks.
Main Results:
- 15 differentially expressed miRNAs were identified in HCHD patients and 20 in ECHD patients compared to healthy controls.
- Eleven pathways were common to both HCHD and ECHD groups; 13 pathways were unique to HCHD and 3 to ECHD.
- hsa-let-7c-5p and hsa-miR-24-3p were suggested to play significant roles in hyperglycemia associated with CHD.
Conclusions:
- Circulating miRNAs differ between hyperglycemic and euglycemic CHD patients.
- Specific miRNAs and pathways are associated with hyperglycemia in the context of CHD.
- hsa-let-7c-5p and hsa-miR-24-3p are potential key players in HCHD.
Abstract:
Coronary heart disease (CHD) has become one of the leading causes of death and functional impairment in the world. Hyperglycemia is associated with an increased risk of cardiovascular disease. It was speculated that miRNAs in peripheral blood were a primary parameter in discriminating CHD. The biological characteristics of coronary heart disease with hyperglycemia (HCHD) and coronary heart disease with euglycemia (ECHD) were investigated in the study. Circulating miRNAs from 26 HCHD patients and 42 ECHD patients were identified by microarrays. Compared with the healthy patients, 15 and 20 differentially expressed miRNAs were identified in HCHD and ECHD groups, respectively. Gene ontology analysis was carried out by DAVID and functional annotations of the miRNA targets related to ATP binding, cellular components, protein binding, RNA binding, DNA binding, and so on. KEGG database was used for pathway analysis. Eleven pathways were identified in both HCHD and ECHD groups. Furthermore, 13 and 3 pathways were only identified in HCHD or ECHD group, respectively. And then, miRNA-gene regulatory networks were constructed to study the relationship between differentially expressed miRNAs and genes. This suggested that hsa-let-7c-5p and hsa-miR-24-3p might have the most important function for hyperglycemia in coronary heart disease patients.
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