Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Feb 17, 2026

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model

Published on: March 9, 2022

[Study of mitochondrial dysfunction using cytoplasmic hybrid].

V V Sinyov, M A Sazonova, V P Karagodin

Patologicheskaia Fiziologiia I Eksperimental'Naia Terapiia

|December 8, 2017

Summary

Cytoplasmic hybrids (cybrids) and mitochondrial DNA-mutated cell lines are valuable models for studying mitochondrial dysfunction. These models reveal cellular pathology in diseases like Alzheimer's and Parkinson's, aiding treatment research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Influence of miR-802 on the Resistance of Non-Small Cell Lung Cancer and Colorectal Cancer Cells to Macrophage Cytotoxic Activity.

Bulletin of experimental biology and medicine·2026

Same author

Diagnostic and Prognostic Significance of RASSF8-AS1 lncRNA Expression in Non-Small Cell Lung Cancer.

Bulletin of experimental biology and medicine·2026

Same author

Clinical Significance of Epigenetic Factors in the Development of Tumor Cell Resistance to the Cytotoxic Activity of Macrophages.

Bulletin of experimental biology and medicine·2025

Same author

Proinflammatory Activation of Monocytes in Patients with Immunoinflammatory Rheumatic Diseases.

Doklady. Biochemistry and biophysics·2024

Same author

Sialic acid as the potential link between lipid metabolism and inflammation in the pathogenesis of atherosclerosis.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2023

Same author

[Association of the structure of the glucocorticoid receptor and single nucleotide <i>NR3C1</i> gene polymorphisms with metabolic disorders].

Problemy endokrinologii·2023

Area of Science:

Cell Biology
Biochemistry
Genetics

Background:

Mitochondrial dysfunction is implicated in numerous human diseases.
Investigating mitochondrial dysfunction requires robust cellular models.

Purpose of the Study:

To review literature on using cytoplasmic hybrids (cybrids) and rho0 cell cultures for studying mitochondrial dysfunction.
To explore the utility of these models in understanding disease mechanisms and potential treatments.

Main Methods:

Analysis of scientific literature focusing on cybrid cell lines (HL60, MOL T-4, A549, 143B, HeLa, Arpe-19, HEK-293, SH-SY5Y, NT2).
Examination of studies investigating biochemical and molecular pathological processes in cybrid cells related to various human diseases.

Main Results:

More Related Videos

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

Analyzing Mitochondrial Morphology Through Simulation Supervised Learning

Analyzing Mitochondrial Morphology Through Simulation Supervised Learning

Published on: March 3, 2023

Related Experiment Videos

Last Updated: Feb 17, 2026

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model

Published on: March 9, 2022

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

Analyzing Mitochondrial Morphology Through Simulation Supervised Learning

Analyzing Mitochondrial Morphology Through Simulation Supervised Learning

Published on: March 3, 2023

Cybrid cell lines and rho0 cultures with mitochondrial genome mutations are promising models for mitochondrial dysfunction research.
These models exhibit consistent functional changes, including reduced oxygen consumption, altered mitochondrial morphology, apoptosis resistance, and impaired respiratory chain complex activity.

Conclusions:

Rho0 cell cultures and cybrid lines with mtDNA mutations serve as effective models for studying mitochondrial genome disorders.
Observed functional similarities between rho0 cells and cybrids compared to native cell lines validate their use in disease research and therapeutic development.