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Updated: Feb 17, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Nitrogen removal performance and microbial community changes in subsurface wastewater infiltration systems (SWISs) at
Chunjing Liu1, Jianzhi Xie1, Manli Song2
1College of Resources and Environmental Science, Hebei Agricultural University, Baoding 071000, PR China; Key Laboratory for Farmland Eco-Environment of Hebei Province, 071000 Baoding, PR China.
Abstract:
Poor nitrogen removal efficiency (mainly nitrate, NO3--N) at low temperatures strongly limits application of subsurface wastewater infiltration systems (SWISs). Seven psychrophilic strains (heterotrophic nitrifying bacteria and aerobic denitrifying bacteria) were isolated and added to SWISs to investigate the effect of embedding and direct-dosing bioaugmentation strategies on sewage treatment performance at low temperature. Both bioaugmentation strategies improved ammonium (NH4+-N) removal efficiencies, and the embedding strategy also exhibited satisfactory NO3--N and total nitrogen (TN) removal efficiencies. Pyrosequencing results of the bacterial 16S rRNA gene indicated that the embedding strategy significantly decreased the indigenous soil microbial diversity (p < .05) and altered the bacterial community structure, significantly increasing the relative abundance of Clostridia, which have good nitrate-reducing activity.
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