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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

8.7K

Decreased circulatory microRNA-4478 as a specific biomarker for diagnosing non-ST-segment elevation myocardial

R Gholikhani-Darbroud, F Khaki-Khatibi, F Mansouri

    Bratislavske Lekarske Listy
    |December 9, 2017
    PubMed
    Abstract

    Insights

    Circulatory microRNA-4478 (miR-4478) and soluble leptin receptor (sLEPR) show promise as diagnostic biomarkers for non-ST-segment elevation myocardial infarction (NSTEMI). Elevated miR-4478 and sLEPR levels were detected in NSTEMI patients, suggesting their potential clinical utility.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cardiology

    Background:

    • MicroRNAs (miRNAs) are increasingly recognized for their potential as predictive biomarkers in various diseases.
    • Circulating miRNAs offer a minimally invasive approach for disease diagnosis and prognosis.

    Purpose of the Study:

    • To evaluate the diagnostic and prognostic utility of circulatory microRNA-4478 (miR-4478) and soluble leptin receptor (sLEPR) in non-ST-segment elevation myocardial infarction (NSTEMI).
    • To establish miR-4478 as a novel biomarker for NSTEMI.
    • To investigate the correlation between miR-4478 and sLEPR, and the effect of miR-4478 on leptin receptor concentration.

    Main Methods:

    • Serum samples were collected from 80 NSTEMI patients and 80 healthy controls.
    • RNA extraction, cDNA synthesis, and quantitative real-time PCR were performed to measure miR-4478 expression.
    • Enzyme-linked immunosorbent assays (ELISA) were used to quantify sLEPR levels, alongside other biochemical markers.

    Main Results:

    • NSTEMI patients exhibited significantly higher serum levels of miR-4478 and sLEPR compared to healthy individuals.
    • A significant positive correlation was observed between miR-4478 and sLEPR levels (R² = 0.698, p < 0.001).
    • miR-4478 demonstrated high sensitivity (87.5%) and specificity (98.8%) for NSTEMI detection, while sLEPR showed 92.5% sensitivity and 87.5% specificity.

    Conclusions:

    • Circulatory miR-4478 and sLEPR are potential predictive biomarkers for NSTEMI.
    • miR-4478 may serve as a novel and effective biomarker for the diagnosis of NSTEMI.
    • The findings support the clinical relevance of miR-4478 and sLEPR in cardiovascular disease diagnostics.
    Keywords:
    Leptin receptormicroRNA-4478non-ST-segment elevation myocardial infarction biomarker.

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