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Effect of lncRNA HOXA11-AS1 on adipocyte differentiation in human adipose-derived stem cells
Nuerbiye Nuermaimaiti1, Jie Liu1, Xiaodi Liang1
1Department of Biochemistry and Molecular Biology, Preclinical Medicine College, Xinjiang Medical University, No. 393 Xinyi Road, Urumqi 830011, Xinjiang, China.
Aims:
To determine the role of lncRNA HOXA11-AS1 on adipocyte differentiation.
Methods:
Human adipose-derived stem cells (hADSCs) were isolated from adipose tissues of patients and cultured in vitro, followed by knockdown of HOXA11-AS1. Then, adipocyte differentiation and expression of adipogenic-related genes (CEBP-α, DGAT2, CIDEC, and perilipin) were measured by RT-qPCR and Western blot.
Results:
We demonstrated that knockdown of HOXA11-AS1 inhibited adipocyte differentiation, leading to suppression of adipogenic-related gene (CEBP-α, DGAT2, CIDEC, and perilipin) transcription, as well as decreased lipid accumulation in hADSCs. In addition, lncRNA HOXA11-AS1 was highly expressed in obese patients and significantly increased during the process of adipocyte differentiation.
Conclusion:
The results provide new insight into the molecular mechanism by which lncRNA HOXA11-AS1 is involved in adipogenesis and may have implications for the treatment of obesity and associated disorders.
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