Related Experiment Video

Updated: Feb 17, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
07:16

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

14.7K

Delta/notch-like epidermal growth factor-related receptor (DNER) orchestrates stemness and cancer progression in

Lijun Wang1, Qi Wu1, Shan Zhu1

  • 1Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan UniversityWuhan, Hubei, P. R. China.

American Journal of Translational Research
|December 9, 2017
PubMed

Insights

Delta/Notch-like epidermal growth factor (EGF)-related receptor (DNER) upregulation promotes prostate cancer progression. Inhibiting DNER in PC-3 cells reduced cancer cell growth, migration, invasion, and tumorigenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Delta/Notch-like epidermal growth factor (EGF)-related receptor (DNER) is a neuron-specific transmembrane protein.
  • The role of DNER in prostate cancer has not been previously investigated.

Purpose of the Study:

  • To investigate the function of DNER in prostate cancer progression.
  • To determine the mechanism by which DNER influences prostate cancer cell growth and characteristics.

Main Methods:

  • Upregulation of DNER was assessed in various cancer types, including prostate cancer.
  • DNER was knocked down in PC-3 prostate cancer cells.
  • Cell proliferation, migration, invasion, and spheroid growth were evaluated.
  • Gene expression analysis (RT-PCR and Western blot) was performed for key cancer stem cell markers (CD44, HES1, GLI1).
  • Tumorigenesis was assessed in PC-3 xenograft models.

Main Results:

  • DNER expression was found to be upregulated in prostate cancer.
  • Knockdown of DNER significantly inhibited PC-3 cell proliferation, migration, and invasion.
  • DNER knockdown suppressed tumor growth in xenograft models and cell growth in spheroids.
  • The expression of CD44, HES1, and GLI1 was significantly decreased in DNER knockdown cells.

Conclusions:

  • DNER plays a crucial role in promoting prostate cancer progression.
  • DNER facilitates prostate cancer cell growth, migration, and invasion.
  • DNER modulates key cancer stem cell genes, contributing to prostate cancer development.
Keywords:
DNERmetastasisproliferationprostate cancerstemness

More Related Videos

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

7.3K
Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel06:44

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

Published on: September 8, 2017

14.1K

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.7K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.5K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.2K
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
6.4K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.7K