Rational lung tissue and animal models for rapid breath tests to determine pneumonia and pathogens
Yong Zhou1, Enguo Chen1, Xiaohong Wu1
1Respiratory Department, Sir Run Run Shaw Hospital, Medical School, Zhejiang UniversityHangzhou, Zhejiang, China.
Objective:
This study works to develop novel models that may be adopted for earlier non-invasive breathomics tests to determine pneumonia pathogens.
Methods:
Two types of pneumonia models were created, both in vitro and in vivo. Paraneoplasm lung tissue and specific pathogen-free (SPF) rabbits were adopted and separately challenged with sterile saline solution control or three pathogens: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. After inoculation, headspace air or exhaled air were absorbed by solid phase micro-extraction (SPME) fibers and subsequently analyzed with gas chromatograph Mass Spectrometer (GCMS).
Results:
Pneumonia and pathogen-specific discriminating VOC patterns (1H-Pyrrole-3-carbonitrile, Diethyl phthalate, Cedrol, Decanoic acid, Cyclohexane, Diisooctyl phthalate) were determined.
Conclusion:
Our study successfully generated nosocomial pneumonia models for pneumonia diagnosis and pathogen-discriminating breath tests. The tests may allow for earlier pneumonia and pathogen diagnoses, and may transfer empirical therapy to targeted therapy earlier, thus improving clinical outcomes.
