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Updated: Feb 17, 2026
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
Published on: July 3, 2017
Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay
Zahra Maria1,2, Véronique A Lacombe3,4
1Department of Physiological Sciences, Oklahoma State University, 283 McElroy Hall, Stillwater, OK, 74078, USA.
Abstract:
The biotinylated photolabeling assay enables quantification of cell-surface glucose transporters (GLUTs). This technique has been successfully applied to quantify the cell-surface GLUT protein content in striated muscles and adipose tissue, as a means to evaluate GLUT trafficking. Here, we describe the detailed method of quantifying the cell-surface content of several GLUT isoforms (1, 4, 8, and 12) in isolated cardiac myocytes, as well as in the intact perfused atria and ventricle.
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