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Multidrug resistant lncRNA profile in chemotherapeutic sensitive and resistant ovarian cancer cells

Juan Xu1, Jiacong Wu2, Chenyang Fu3

  • 1Department of Obstetrics and Gynecology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.

Journal of Cellular Physiology
|December 9, 2017
PubMed

Insights

Researchers identified key long non-coding RNAs (lncRNAs) involved in multidrug resistance in ovarian cancer. These findings offer insights into overcoming treatment resistance in cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ovarian cancer often develops acquired multidrug resistance (MDR), leading to treatment failure.
  • The precise mechanisms underlying MDR in ovarian cancer remain incompletely understood.
  • Long non-coding RNAs (lncRNAs) are emerging as critical regulators in various biological processes, including drug resistance.

Purpose of the Study:

  • To identify and characterize lncRNAs associated with multidrug resistance in ovarian cancer.
  • To investigate the expression patterns of potential MDR-related lncRNAs in resistant cell lines and patient data.
  • To explore the functional roles of MDR-related lncRNAs by predicting their mRNA targets.

Main Methods:

  • LncRNA sequencing was performed on the ovarian cancer cell line A2780 and its paclitaxel-resistant counterpart (A2780/PTX).
  • Publicly available datasets of cisplatin-resistant ovarian cancer cell lines and patients were integrated to identify MDR lncRNAs.
  • Real-time PCR was used to validate the expression of selected MDR lncRNAs in resistant ovarian and colon cancer cell lines.
  • An lncRNA-mRNA co-expression network was constructed to predict potential targets of MDR lncRNAs.

Main Results:

  • Five up-regulated and 21 down-regulated lncRNAs were identified as potentially associated with multidrug resistance.
  • Real-time PCR confirmed similar expression changes for 5 up-regulated and 4 down-regulated MDR lncRNAs in both ovarian and colon cancer cell lines.
  • The multidrug resistance genes ABCB1, ABCB4, ABCC3, and ABCG2 were found to be co-expressed with lncRNA CTD-2589M5.4.

Conclusions:

  • This study identifies novel lncRNAs implicated in the multidrug resistance of ovarian cancer.
  • The findings highlight the potential role of specific lncRNAs, such as CTD-2589M5.4, in regulating MDR genes.
  • The identified MDR lncRNAs provide valuable targets for understanding and potentially overcoming chemoresistance in ovarian cancer.
Keywords:
LncRNAcisplatinepirubicinmultidrug resistanceovarian cancerpaclitaxel

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