Mucilaginibacter amnicola sp. nov., isolated from a freshwater creek

A pink-coloured bacterial strain, TAPP7^T, was isolated from a freshwater creek in Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TAPP7^T belonged to the genus Mucilaginibacter and showed highest similarity with Mucilaginibacter ginsengisoli B4Y-8^T (97.6 %) and Mucilaginibacter carri PR0008K^T (96.9 %). Cells of strain TAPP7^T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate-accumulating and short-rod-shaped. Growth occurred at 10-30 °C (optimum, 15-20 °C), at pH 4-8 (optimum, pH 6) and with 0-1 % NaCl (optimum, 0.5 %). The predominant fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C15 : 0. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The major isoprenoid quinone was MK-7. The DNA G+C content of the genomic DNA was 45.6 mol%. The DNA-DNA relatedness of strain TAPP7^T with respect to Mucilaginibacter ginsengisoli B4Y-8^T was less than 35 %. On the basis of the phylogenetic inference and phenotypic data, strain TAPP7^T should be classified as a novel species, for which the name Mucilaginibacter amnicola sp. nov. is proposed. The type strain is TAPP7^T (=BCRC 80976^T=LMG 29556^T=KCTC 52238^T).