Mesophyll conductance in Zea mays responds transiently to CO₂ availability: implications for transpiration efficiency...

Mesophyll conductance (g_m ) describes the movement of CO₂ from the intercellular air spaces below the stomata to the site of initial carboxylation in the mesophyll. In contrast with C₃ -g_m , little is currently known about the intraspecific variation in C₄ -g_m or its responsiveness to environmental stimuli. To address these questions, g_m was measured on five maize (Zea mays) lines in response to CO₂ , employing three different estimates of g_m . Each of the methods indicated a significant response of g_m to CO₂ . Estimates of g_m were similar between methods at ambient and higher CO₂ , but diverged significantly at low partial pressures of CO₂ . These differences are probably driven by incomplete chemical and isotopic equilibrium between CO₂ and bicarbonate under these conditions. Carbonic anhydrase and phosphoenolpyruvate carboxylase in vitro activity varied significantly despite similar values of g_m and leaf anatomical traits. These results provide strong support for a CO₂ response of g_m in Z. mays, and indicate that g_m in maize is probably driven by anatomical constraints rather than by biochemical limitations. The CO₂ response of g_m indicates a potential role for facilitated diffusion in C₄ -g_m . These results also suggest that water-use efficiency could be enhanced in C₄ species by targeting g_m .