Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
Ribosome Profiling02:24

Ribosome Profiling

4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Cell‑autonomous IL6ST activation suppresses prostate cancer development via STAT3/ARF/p53‑driven senescence and confers an immune‑active tumor microenvironment.

Molecular cancer·2026
Same author

Lack of evidence for the presence of plastids in the evolutionary history of kinetoplastid protists.

Folia parasitologica·2026
Same author

Leishmania major virulence attenuation in vitro: An old conundrum revisited in the omics era.

PLoS neglected tropical diseases·2026
Same author

Volume electron microscopy reveals bacterial endosymbiosis within host mitochondria.

Communications biology·2026
Same author

Trypanosomes of fish as an emerging threat to aquaculture systems in China.

PLoS pathogens·2026
Same author

Frequent occurrence and predicted functions of tRNAs with 4-base-pair anticodon stems in bacteria: extended superwobble hypothesis.

Nucleic acids research·2026

Related Experiment Video

Updated: Feb 17, 2026

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

8.9K

Trypanosomatid mitochondrial RNA editing: dramatically complex transcript repertoires revealed with a dedicated

Evgeny S Gerasimov1,2, Anna A Gasparyan1, Iosif Kaurov3,4

  • 1Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow 119991, Russia.

Nucleic Acids Research
|December 9, 2017
PubMed
Summary

Analyzing kinetoplastid mitochondrial RNA editing, this study introduces T-Aligner, a new tool for comprehensive transcript analysis. It reveals complex editing patterns, including alternative open reading frames, in Leptomonas pyrrhocoris and Trypanosoma cruzi.

More Related Videos

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
07:26

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

6.5K
Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K

Related Experiment Videos

Last Updated: Feb 17, 2026

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

8.9K
Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
07:26

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

6.5K
Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • RNA editing, specifically uridine insertion and deletion, is essential for generating translatable messenger RNAs (mRNAs) from mitochondrial cryptogenes in kinetoplastids.
  • This editing process is complex, often involving more inserted/deleted uridines than genome-encoded nucleotides, making transcriptome analysis challenging.

Purpose of the Study:

  • To develop and present T-Aligner, a novel computational tool for comprehensive characterization of RNA editing in kinetoplastid mitochondrial transcriptomes.
  • To analyze deep sequencing data from Leptomonas pyrrhocoris and Trypanosoma cruzi to understand complex RNA editing events.

Main Methods:

  • Deep sequencing of mitochondrial cDNA from Leptomonas pyrrhocoris.
  • Application of T-Aligner, a new tool for read mapping, visualization of editing states, and assembly of canonical and alternative mRNAs.
  • Validation of T-Aligner using deep sequencing reads from Trypanosoma cruzi.

Main Results:

  • T-Aligner enables comprehensive RNA editing characterization without targeted amplification or prior knowledge of edited products.
  • Analysis revealed highly complex RNA editing in Leptomonas pyrrhocoris and Trypanosoma cruzi mitochondrial transcripts.
  • Complex editing includes the generation of alternative open reading frames and truncated mRNA products.

Conclusions:

  • T-Aligner is an effective tool for detailed analysis of complex RNA editing in kinetoplastid mitochondrial transcriptomes.
  • Kinetoplastid mitochondrial RNA editing is intricate, leading to diverse mRNA products including alternative reading frames and truncated forms.