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Stimuli-activated drug delivery systems are designed to release drugs in response to specific physical, chemical, or biological stimuli. These systems often utilize hydrogels—three-dimensional, hydrophilic polymer networks capable of swelling in aqueous environments and retaining significant fluid volumes. Upon exposure to particular stimuli, these hydrogels undergo structural transitions that allow the embedded drug to be released. Due to this adaptive behavior, such systems are also...
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Dual-Gated Microparticles for Switchable Antibody Release.

Benedikt Ketterer1, Huey Wen Ooi1,2, Dominik Brekel1

  • 1Institute for Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT) , Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.

ACS Applied Materials & Interfaces
|December 9, 2017
PubMed
Summary

We developed novel dual-gated microparticles for efficient antibody separation using stimuli-responsive chromatography. These microparticles bind antibodies at 40°C/pH 8 and release them at 5°C or pH 3, enabling easy column regeneration.

Keywords:
column chromatographypH responsive microparticlespolymer graftingprotein separationthermo-responsive polymer microparticles

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Area of Science:

  • Biomaterials Science
  • Chromatography
  • Polymer Chemistry

Background:

  • Stimuli-responsive materials offer tunable properties for advanced separation techniques.
  • Antibody purification is critical in biopharmaceutical development, requiring efficient and selective methods.

Purpose of the Study:

  • To design and synthesize dual-gated microparticles responsive to both temperature and pH for stimuli-responsive chromatography.
  • To achieve efficient separation and purification of antibodies using these novel microparticles.

Main Methods:

  • Synthesis of dual-gated microspheres via RAFT-based thiol-terminal block copolymers grafted onto poly(glycidyl methacrylate) (PGMA) microparticles.
  • Utilizing 4-vinylpyridine units for protein binding and stimuli-responsive release triggered by temperature and pH changes.

Main Results:

  • Demonstrated switchable protein release of immunoglobulins by altering temperature (40°C to 5°C) or pH (8 to 3).
  • Achieved a maximum immunoglobulin adsorption capacity of 18.9 mg mL⁻¹ with P(NIPAM₂₉-b-4VP₃₀)-grafted PGMA particles at 40°C/pH 8.
  • Showcased facile column unloading via temperature switch and efficient regeneration by lowering pH to 3.

Conclusions:

  • Dual-gated microparticles provide a promising platform for stimuli-responsive chromatography.
  • The developed system enables efficient antibody separation, purification, and facile regeneration of chromatographic columns.