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Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
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Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

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MPK1/SLT2 Links Multiple Stress Responses with Gene Expression in Budding Yeast by Phosphorylating Tyr1 of the RNAP

Nathan Yurko1, Xiaochuan Liu2, Takashi Yamazaki1

  • 1Department of Biological Sciences, Columbia University, New York, NY 10027, USA.

Molecular Cell
|December 9, 2017
PubMed

Insights

The study reveals that substituting tyrosine-1 (Tyr1) in RNA polymerase II

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The C-terminal domain of RNA polymerase II's largest subunit features YSPTSPS repeats.
  • The function of tyrosine-1 (Tyr1) in this domain is not fully understood.
  • Previous studies show varied effects of Tyr1 mutations across species.

Purpose of the Study:

  • To investigate the role of Tyr1 in budding yeast.
  • To explore the connection between Tyr1, cellular stress, and MAP kinase signaling.
  • To identify the kinase responsible for Tyr1 phosphorylation.

Main Methods:

  • Generating and analyzing a Tyr1 to Phenylalanine (Y1F) mutant in budding yeast.
  • Assessing growth phenotypes and stress sensitivity.
  • Investigating transcriptional changes and genetic/biochemical interactions.
  • Performing in vitro and in vivo phosphorylation assays.

Main Results:

  • The Y1F mutation caused slow growth and hypersensitivity to cellular stresses.
  • Phenotypes were associated with transcriptional alterations.
  • Genetic and biochemical interactions between Tyr1 and transcription factors were observed.
  • The MAP kinase Slt2 was identified as a Tyr1 kinase, phosphorylating Tyr1 in vitro and in vivo.

Conclusions:

  • Tyr1 phosphorylation by Slt2 is crucial for budding yeast.
  • This finding links stress response pathways to Tyr1 function in RNA polymerase II.
  • The study elucidates a novel regulatory mechanism for transcription.
Keywords:
MediatorNNS complexRNA polymerase IISlt2 kinasestress responsetranscription terminationtyrosine phosphorylation

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