Biodegradation and detoxification of Direct Black G textile dye by a newly isolated thermophilic microflora
Yan Chen1, Linlin Feng1, Hanguang Li1
1College of Bioscience and Biotechnology, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, PR China; Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, Jiangxi Agricultural University, Nanchang 330045, PR China.
Abstract:
The biodegradation and detoxification of azo dye - Direct Black G (DBG) with a newly isolated thermophilic microflora was investigated in the present study. It was found this microflora can decolorize DBG at a wide range of pH from 5 to 10, and grow well under high concentration of dye (600 mg·L-1) and salinity (50 g·L-1). Its decolorization ratio could reach 97% with 8 h of incubation at optimal conditions. The induction of laccase, manganese peroxidase, lignin peroxidase and azoreductase suggests their synergetic involvements in the degradation process of DBG. In addition, the phytotoxicity analysis indicated the thermophilic microflora converted toxic dye DBG into low toxicity metabolites. PCR-DGGE analysis revealed that there are nine different bacteria presented in this microflora. Furthermore, a new degradation pathway of DBG degradation by this microflora was proposed based on the intermediates identified by LC-ESI-MS/MS.
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