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Integrated Host-Pathogen Metabolic Reconstructions.

Anu Raghunathan1, Neema Jamshidi2,3

  • 1Chemical Engineering Division, National Chemical Laboratory, Pune, India. anu.raghunathan@ncl.res.in.

Methods in Molecular Biology (Clifton, N.J.)
|December 10, 2017
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Summary
This summary is machine-generated.

This study presents a protocol for merging host and pathogen metabolic networks to model infectious diseases. This systems biology approach aids in understanding complex host-pathogen interactions and predicting disease outcomes.

Keywords:
Constraints-based analysisFlux balance analysisHost pathogen integrated modelStoichiometric merge

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Area of Science:

  • Systems Biology
  • Metabolic Network Modeling
  • Infectious Disease Research

Background:

  • Genome-scale metabolic reconstructions are established for diverse organisms.
  • Constraints-based models link genotype to metabolic phenotypes.
  • Infectious diseases involve complex host-pathogen multifactorial responses.

Purpose of the Study:

  • To describe a protocol for integrating host and pathogen metabolic networks.
  • To facilitate the analysis of host-pathogen interactions using merged stoichiometric matrices.
  • To provide a framework for understanding emergent properties in infectious disease systems.

Main Methods:

  • Detailed protocol for merging metabolic networks and stoichiometric matrices.
  • Utilized a Salmonella-mouse macrophage model for demonstration.
  • Discussion of interfacial and objective functions for model analysis.

Main Results:

  • A standardized protocol for host-pathogen metabolic network integration.
  • Demonstration of merging stoichiometric matrices for a specific model system.
  • Identification of necessary functions for analyzing host-pathogen interactions.

Conclusions:

  • The developed protocol simplifies the complex process of integrating host and pathogen metabolic data.
  • This approach enables more accurate modeling and prediction of infectious disease dynamics.
  • Facilitates deeper understanding of host-pathogen metabolic interplay in disease.