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Culture of Rodent Cortical, Hippocampal, and Striatal Neurons
Laura Facci1, Stephen D Skaper2
1Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|December 10, 2017
Abstract:
Neurons cultured from rodent central nervous system tissues represent important tools in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical, hippocampal, and striatal neuron cell cultures, using either embryonic or postnatal tissue with enzymatic digestion.
