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Updated: Feb 17, 2026

Author Spotlight: In Vitro Co-Culture Model for Studying Microglia-Neuronal Interactions in Disease Conditions
Published on: July 26, 2024
Central Nervous System Neuron-Glia co-Culture Models and Application to Neuroprotective Agents
Stephen D Skaper1, Laura Facci2
1Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy. stephen.skaper@unipd.it.
Abstract:
Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders. This article presents a protocol for the preparation of cultures consisting of rat embryonic cortical neurons grown in the presence of cortical microglia, in which the glia are present in physical contact with the neurons or separated by a semipermeable membrane barrier. An example of how such systems can be used to evaluate potential neuroprotective agents will also be described.
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