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Updated: Feb 17, 2026
Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
Published on: August 16, 2018
Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses
Dimitar Dimitrov1, Laurent Guillaud2, Kohgaku Eguchi3
1Cellular and Molecular Synaptic Function Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan. ddimitrov@oist.jp.
Abstract:
Primary neuronal cell culture preparations are widely used to investigate synaptic functions. This chapter describes a detailed protocol for the preparation of a neuronal cell culture in which giant calyx-type synaptic terminals are formed. This chapter also presents detailed protocols for utilizing the main technical advantages provided by such a preparation, namely, labeling and imaging of synaptic organelles and electrophysiological recordings directly from presynaptic terminals.
