Oxidative stress in birds along a NO_x and urbanisation gradient: An interspecific approach

Urbanisation is regarded as one of the most threatening global issues for wildlife, however, measuring its impact is not always straight forward. Oxidative stress physiology has been suggested to be a useful biomarker of health and therefore, a potentially important indicator of the impact that urban environmental stressors, especially air pollution, can have on wildlife. For example, nitrogen oxides (NO_x), released during incomplete combustion of fossil fuels, are highly potent pro-oxidants, thus predicted to affect either the protective antioxidants and/or cause oxidative damage to bio-molecules. To date, epidemiological modelling of the predicted association between oxidative stress and NO_x exposure has not been performed in wild animals. Here, we address this short-coming, by investigating multiple oxidative stress markers in four common passerine bird species, the blue tit (Cyanistes caeruleus), great tit (Parus major), house sparrow (Passer domesticus) and tree sparrow (Passer montanus), living along a gradient of NO_x and urbanisation levels in southern Sweden. First of all, the results revealed that long- and medium-term (one month and one week, respectively) NO_x levels were highly correlated with the level of urbanisation. This confirms that the commonly used urbanisation index is a reliable proxy for urban air pollution. Furthermore, in accordance to our prediction, individuals exposed to higher long- and medium-term NO_x levels/urbanisation had higher plasma antioxidant capacity. However, only tree sparrows showed higher oxidative damage (protein carbonyls) in relation to NO_x levels and this association was absent with urbanisation. Lipid peroxidation, glutathione and superoxide dismutase levels did not co-vary with NO_x/urbanisation. Given that most oxidative stress biomarkers showed strong species-specificity, independent of variation in NO_x/urbanisation, the present study highlights the need to study variation in oxidative stress across contexts, seasons and life-stages in order to understand how the ecology and phylogeny of species interact to affect species resilience to urban environmental stress.