Related Experiment Video
Updated: Feb 17, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
Published on: October 28, 2014
An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome
Hyun Yong Jin1, Changchun Xiao2
1Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, R202, La Jolla, CA, 92037, USA.
Abstract:
Recent advances in global translatome analysis technologies enable us to understand how translational regulation of gene expression modulates cellular functions. In this chapter, we present an integrated method to measure various aspects of translatome by polysome profiling and ribosome profiling using purified B cells. We standardized our protocols to directly compare the results from these two approaches. Parallel assessment of translatome with these two approaches can generate a comprehensive picture on how translational regulation determines protein output.
Related Concept Videos
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Proteomics
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Translation in Prokaryotes

