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Updated: Feb 17, 2026

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
Published on: October 14, 2022
Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells
Xiangzhi Meng1, Nicole Riley2, Ryan Thompson3
1Department of Molecular Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, SR-111, La Jolla, CA, 92037, USA. xmeng@scripps.edu.
Abstract:
Hi-C is a methodology developed to reveal chromosomal interactions from a genome-wide perspective. Here, we described a protocol for generating Hi-C sequencing libraries in resting and activated human naive CD4 T cells to investigate activation-induced chromatin structure re-arrangement in T cell activation followed by a section reviewing the general concepts of Hi-C data analysis.
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