Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An integrated molecular risk score early in life for subsequent childhood asthma risk.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2024
Same author

Circulating metabolites modulated by diet are associated with depression.

Molecular psychiatry·2023
Same author

Network reconstruction for trans acting genetic loci using multi-omics data and prior information.

Genome medicine·2022
Same author

The German National Pandemic Cohort Network (NAPKON): rationale, study design and baseline characteristics.

European journal of epidemiology·2022
Same author

Integrative analysis of clinical and epigenetic biomarkers of mortality.

Aging cell·2022
Same author

17q12-21 risk-variants influence cord blood immune regulation and multitrigger-wheeze.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2021

Related Experiment Video

Updated: Feb 17, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.5K

Quantitative Region-Specific DNA Methylation Analysis by the EpiTYPER™ Technology.

Sonja Kunze1

  • 1Research Unit of Molecular Epidemiology/Institute of Epidemiology II, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr. 1, 85764, Neuherberg, Germany. sonja.kunze@helmholtz-muenchen.de.

Methods in Molecular Biology (Clifton, N.J.)
|December 11, 2017
PubMed
Summary

The EpiTYPER™ assay provides high-resolution, quantitative DNA methylation analysis for targeted regions. This method enables detailed study of single CpG sites and validation of genome-wide findings, advancing epigenome research.

Keywords:
Base-specific cleavageBisulfiteDNA methylation patternEpiTYPERMALDI-TOF MSQuantitative

More Related Videos

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.1K
Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

19.4K

Related Experiment Videos

Last Updated: Feb 17, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.5K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.1K
Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

19.4K

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genomics

Background:

  • DNA methylation is crucial for development, health, and disease progression.
  • High-throughput DNA methylation analysis is vital for epigenome research.
  • Genome-wide techniques often provide limited data on individual CpG sites within regions.

Purpose of the Study:

  • To introduce and evaluate the EpiTYPER™ assay for region-specific DNA methylation analysis.
  • To enable high-resolution scanning and detailed analysis of selected genomic regions.
  • To validate findings from genome-wide methylation studies.

Main Methods:

  • The EpiTYPER™ assay utilizes base-specific cleavage of bisulfite-converted DNA.
  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is employed for detection.
  • The method allows for targeted quantification of individual CpG sites in a high-throughput manner.

Main Results:

  • The EpiTYPER™ assay can analyze up to 85% of CpGs within a target region.
  • It achieves high detection precision, quantifying methylation differences as low as 5-7%.
  • The assay is fast and reproducible for targeted DNA methylation quantification.

Conclusions:

  • The EpiTYPER™ assay offers a powerful tool for detailed, region-specific DNA methylation analysis.
  • It complements genome-wide approaches by enabling validation and in-depth investigation of CpG sites.
  • This method advances the study of epigenome dynamics in health and disease.