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A Reduced Transcriptome Approach to Assess Environmental Toxicants Using Zebrafish Embryo Test.

Pingping Wang1, Pu Xia1, Jianghua Yang1

  • 1State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University , Nanjing, P. R. China , 210023.

Environmental Science & Technology
|December 12, 2017
PubMed
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This summary is machine-generated.

A new reduced zebrafish transcriptome (RZT) approach offers a cost-effective method for toxicogenomic analysis. This sensitive tool accurately profiles chemical bioactivity and prioritizes toxicants by identifying key biological pathways.

Area of Science:

  • Environmental Toxicology
  • Genomics
  • Molecular Biology

Background:

  • Omics approaches monitor biological responses to environmental toxicants but are limited by high costs and lack of standardized toxicogenomic methods.
  • Transcriptomics offers genome-scale insights but requires efficient, cost-effective tools for broad chemical assessment.

Purpose of the Study:

  • To develop and validate a reduced zebrafish transcriptome (RZT) approach for cost-effective toxicogenomic profiling.
  • To assess the bioactivity of chemicals and environmental mixtures using zebrafish embryos.
  • To establish a sensitive method for prioritizing toxicants based on pathway responsiveness.

Main Methods:

  • Developed a reduced zebrafish transcriptome (RZT) gene set (1637 Entrez genes) to represent whole transcriptome changes.

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  • Utilized RNA-ampliseq technology for quantitative gene expression analysis in zebrafish embryos.
  • Applied concentration-response modeling to determine effect concentrations (ECs) of differentially expressed genes (DEGs) and pathways.

    • Main Results:

    • The RZT-ampliseq-embryo approach demonstrated sensitivity in identifying a wide spectrum of biological activities from BPA exposure and water samples.
    • Water quality was benchmarked using a sensitivity distribution curve of biological pathways.
    • Identified key pathways linked to reproductive outcomes, genotoxicity, and developmental effects.

    Conclusions:

    • The RZT-ampliseq-embryo approach provides an efficient and cost-effective tool for toxicogenomic assessment.
    • This method enables accurate profiling of chemical bioactivity and prioritization of toxicants based on sensitive biological pathway responses.
    • The approach is valuable for monitoring environmental toxicants and assessing potential adverse effects.