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Oncogenic non-coding RNA NEAT1 promotes the prostate cancer cell growth through the SRC3/IGF1R/AKT pathway

Wei Xiong1, Changkun Huang1, Huanghao Deng1

  • 1Department of Urology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.

The International Journal of Biochemistry & Cell Biology
|December 12, 2017
PubMed

Insights

Nuclear Enriched Abundant Transcript 1 (NEAT1) interacts with Steroid Receptor Coactivator 3 (SRC3) to promote prostate cancer growth. This NEAT1/SRC3 interaction upregulates the IGF1R/AKT pathway, driving cancer progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Steroid receptor coactivator 3 (SRC3) is an androgen receptor (AR) coactivator implicated in prostate cancer progression.
  • Non-coding RNAs (ncRNAs) are increasingly recognized for their roles in cancer development, but their interaction with AR coactivators remains poorly understood.

Purpose of the Study:

  • To investigate the functional relationship between ncRNA and SRC3 in prostate cancer.
  • To identify specific ncRNAs that interact with SRC3 and elucidate their mechanism of action in prostate cancer cell growth.

Main Methods:

  • Prostate cancer cell lines were used to study the interaction between NEAT1 and SRC3.
  • Western blotting and cell proliferation assays were employed to analyze the effects of NEAT1 on the SRC3/IGF1R/AKT pathway.
  • Expression levels of NEAT1, SRC3, and IGF1R were examined in patient samples.

Main Results:

  • Nuclear Enriched Abundant Transcript 1 (NEAT1) was found to interact with SRC3 in prostate cancer cells.
  • NEAT1 upregulates AKT phosphorylation through the SRC3/IGF1R pathway, thereby promoting prostate cancer cell growth.
  • Elevated expression of NEAT1, SRC3, and IGF1R was observed in prostate cancer patient samples.

Conclusions:

  • NEAT1 is identified as a novel SRC3-binding ncRNA.
  • NEAT1 promotes prostate cancer cell proliferation via the SRC3/IGF1R/AKT signaling pathway.
  • Targeting the NEAT1/SRC3/IGF1R/AKT axis may offer a therapeutic strategy for prostate cancer.
Keywords:
IGF1RNEAT1Prostate cancerSRC3

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