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Updated: Feb 17, 2026

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AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
Published on: June 23, 2023
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A Proposal for Automated Background Removal of Bio-Raman Data
Tatsuro Sugawara1, Qi Yang1, Takakazu Nakabayashi1
1Graduate School of Pharmaceutical Sciences, Tohoku University.
Abstract:
The subtraction of background components from observed spectra is essential in performing multivariable analysis, frequently applied to Raman spectra of cells. The subtraction procedure, however, is manual and time consuming, especially for big data. Here, we propose an automated method for removing background information from measured spectra of cells, exhibiting the theoretical framework and an experimental application.

