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Updated: Feb 17, 2026

Massively Parallel Reporter Assays in Cultured Mammalian Cells
Published on: August 17, 2014
New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
Séverine A Degrelle1,2,3, Hussein Shoaito1,2, Thierry Fournier1,2,3
1INSERM, UMR-S1139, Faculté de Pharmacie de Paris, 75006 Paris, France.
Abstract:
The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPARγ activity in different cell types and screen for PPARγ's potential ligands.

